This work leads to the molecular understanding of the role RACK1A plays in the auxin induced lateral root development signaling pathways. Taken together, auxin induced RACK1A Tyr 248 phosphorylation is found to be the critical regulatory mechanism for auxin-mediated lateral root development. In contrast, two other compounds, SD29 and SD29-14, inhibited auxin induced RACK1A Tyr 248 phosphorylation resulting in the inhibition of auxin sensitivity and alternation in the lateral roots formation. Through monitoring the auxin response in the architecture of lateral roots and auxin reporter assays, a small molecule- SD29-12 was found to stabilize the auxin induced RACK1A Tyr 248 phosphorylation, thereby stimulating auxin signaling and inducing lateral roots formation. Here, the three different small compounds are used to elucidate the role of RACK1A in auxin mediated lateral root development. Previously, a group of small compounds targeting the Arabidopsis RACK1A functional site-Tyr 248 have been developed.
Many reports implicated RACK1 in plant hormone signal transduction pathways including in auxin and diverse stress signaling pathways however, the precise molecular mechanism of its role is not understood. Receptor for activated C kinase 1 (RACK1) is WD-40 type scaffold protein, conserved in all eukaryote organisms.
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